Acute contact toxicity test (honeybees)

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Term Acute contact toxicity test (honeybees)
Description
Definition Acute contact toxicity: is the adverse effects occurring within a maximum period of 96 h of a topical application of a single dose of a substance.

Adult worker honeybees (Apis mellifera) are exposed to a range of doses of the test substance dissolved in appropriate carrier, by direct application to the thorax (or oplets). The test duration is 48 h. If the mortality rate is increasing between 24 h and 48 h whilst control mortality remains at an accepted level, i.e. < 10 %, it is appropriate to extend the duration of the test to a maximum of 96 h. Mortality is recorded daily and compared with control values. The results are analysed in order to calculate the LD 50 at 24 h and 48 h, and in case the study is prolonged at 72 h and 96 h.

References 1. http://ecb.jrc.ec.europa.eu/documents/Testing-Methods/ANNEXV/C17web2001.pdf
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